Single Step Precision Genome Editing in Yeast using CRISPR-Cas9
We recently published a protocol on Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9. This protocol can be used to precisely modify any location in the yeast genome (essential or non-essential) without the need for intermnediate strains carrying antibiotic or auxotrophic markers. We successfully demonstrated the protocol in our recent publication Systematic Bacterilazation of Yeast Genes by functionally replacing essential yeast genes with their bacterial or human orthologs. This protocol can be used to add N-terminal or C-terminal tags and introduce site-specific muations in yeast genes using appropriate repair templates (HDR-Homology Directed Repair) or simply just randomly mutating yeast genes (via NHEJ-Non-Homologous End Joining) without any repair template.
New students joining the lab
Welcome! Mudabir Abdullah, a Masters student from the University of Kashmir. He did his post Master’s Research fellowship at the Indian Institute of Science, Bangalore under the supervision of Prof. Sandhya S. Visweswariah. Welcome Mudabir!
New students joining the lab
Our Lab is recruiting.
Kachroo lab is looking for talented graduate students. The selected candidates will be supported by funding from the Faculty of Arts and Sciences at Concordia University. The prospective students will work at Canada’s first Genome Foundry for Synthetic Biology research. Check the advertisement here. And follow the instrutions for application here.
Bacterialization of yeast genes
In June 2017, we published our work on systematic bacterialization of essential genes in baker’s yeast in eLife journal. This work showed that many essential yeast genes can be replaced by their bacterial counterparts, despite over 2 billion years of evolutionary divergence between prokaryotes and eukaryotes identifying ancient heme biosynthesis as a universally swappable pathway.
Humanization of yeast genes
In 2015, we published an article on systematic replacement of essential genes in baker’s yeast with corresponding human orthologs. This work demonstrated that nearly 50% of the essential yeast genes can be replaced by their human counterparts, despite over a billion years of evolutionary divergence between humans and yeast. We discovered that metabolic pathways and protein complexes were either entirely replaceable or not.
My lab at Concordia university will expand on this work to fully humanize genetic systems in yeast.
This article was highlighted at many news and other media outlets, links to which are provided below:
Other news features on this paper: phys.org – Discovery News – The Scientist – Science daily – MeteoWeb (in Italian) – NBC News – Washington Post – Reddit #1 – Science News – Empire State Tribune – Softpedia – Reddit #2 – Daily Mail – redOrbit – Medical Daily – NYC Today – Genetic Literacy Project – Techie News – Tech Times – American Live Wire – Pioneer News – Yahoo News – IFLScience – yeastgenome.org – Ottawa Citizen – PRI