We recently published a protocol on Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9. This protocol can be used to precisely modify any location in the yeast genome (essential or non-essential) without the need for intermnediate strains carrying antibiotic or auxotrophic markers. We successfully demonstrated the protocol in our recent publication Systematic Bacterilazation of Yeast Genes by functionally replacing essential yeast genes with their bacterial or human orthologs. This protocol can be used to add N-terminal or C-terminal tags and introduce site-specific muations in yeast genes using appropriate repair templates (HDR-Homology Directed Repair) or simply just randomly mutating yeast genes (via NHEJ-Non-Homologous End Joining) without any repair template.
Welcome! Mudabir Abdullah, a Masters student from the University of Kashmir. He did his post Master’s Research fellowship at the Indian Institute of Science, Bangalore under the supervision of Prof. Sandhya S. Visweswariah. Welcome Mudabir!
Kachroo lab is looking for talented graduate students. The selected candidates will be supported by funding from the Faculty of Arts and Sciences at Concordia University. The prospective students will work at Canada’s first Genome Foundry for Synthetic Biology research. Check the advertisement here. And follow the instrutions for application here.